il6 neutralizing antibodies Search Results


94
Sino Biological antibody mab 206
Antibody Mab 206, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sino Biological il6
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Sino Biological 10395 r508
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Diaclone neutralizing antibodies to interleukin-6 (il-6) (b-e8)
Neutralizing Antibodies To Interleukin 6 (Il 6) (B E8), supplied by Diaclone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson neutralizing anti-il-6 ab
Neutralizing Anti Il 6 Ab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson il-6 neutralization antibody
A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) <t>factor</t> <t>IL-6</t> was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).
Il 6 Neutralization Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson neutralizing monoclonal antibodies human il-6, il-8, vegf
A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) <t>factor</t> <t>IL-6</t> was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).
Neutralizing Monoclonal Antibodies Human Il 6, Il 8, Vegf, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson neutralizing anti-human il-7 antibody
A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) <t>factor</t> <t>IL-6</t> was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).
Neutralizing Anti Human Il 7 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bioceros Inc il6-neutralizing antibodies
A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) <t>factor</t> <t>IL-6</t> was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).
Il6 Neutralizing Antibodies, supplied by Bioceros Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pfizer Inc neutralizing il-6 antibodies for human
A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) <t>factor</t> <t>IL-6</t> was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).
Neutralizing Il 6 Antibodies For Human, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mabtech Inc neutralizing antibodies to il-6
A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) <t>factor</t> <t>IL-6</t> was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).
Neutralizing Antibodies To Il 6, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PeproTech anti-rat il-6 neutralizing antibody 500-p73
A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) <t>factor</t> <t>IL-6</t> was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).
Anti Rat Il 6 Neutralizing Antibody 500 P73, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) factor IL-6 was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).

Journal: Cell reports

Article Title: Stromal-initiated changes in the bone promote metastatic niche development

doi: 10.1016/j.celrep.2015.12.016

Figure Lengend Snippet: A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) factor IL-6 was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).

Article Snippet: For IL-6 neutralization in this assay, senescent or non-senescent osteoblasts were co-cultured with BMMs and 5pg/μl of IL-6 neutralization antibody (Cat#554400, BD Biosciences, San Jose, CA 95131) or 5pg/μl of IgG control (Cat#40041B, BioLegend, San Diego, CA 92121) was added to the medium.

Techniques: Staining, Two Tailed Test, Quantitative RT-PCR

A. Quantification of osteoclasts following co-culture with non-senescent (NS) or senescent (SEN) osteoblasts in the presence of a control (IgG) or IL-6 neutralizing antibody (α-IL-6). Bone marrow derived macrophages were cultured with non-senescent or senescent osteoblasts for 4 days in the presence of 5 pg α-IL-6. The antibody was replaced every other day. TRAP staining was carried out after 4 days co-culture and osteoclast numbers were quantitated and are displayed as TRAP-positive osteoclasts per field of view (TRAP+OC/FOV) (SEM, *p<0.05, two-tailed, Student t test; one of two representative experiment is shown).

Journal: Cell reports

Article Title: Stromal-initiated changes in the bone promote metastatic niche development

doi: 10.1016/j.celrep.2015.12.016

Figure Lengend Snippet: A. Quantification of osteoclasts following co-culture with non-senescent (NS) or senescent (SEN) osteoblasts in the presence of a control (IgG) or IL-6 neutralizing antibody (α-IL-6). Bone marrow derived macrophages were cultured with non-senescent or senescent osteoblasts for 4 days in the presence of 5 pg α-IL-6. The antibody was replaced every other day. TRAP staining was carried out after 4 days co-culture and osteoclast numbers were quantitated and are displayed as TRAP-positive osteoclasts per field of view (TRAP+OC/FOV) (SEM, *p<0.05, two-tailed, Student t test; one of two representative experiment is shown).

Article Snippet: For IL-6 neutralization in this assay, senescent or non-senescent osteoblasts were co-cultured with BMMs and 5pg/μl of IL-6 neutralization antibody (Cat#554400, BD Biosciences, San Jose, CA 95131) or 5pg/μl of IgG control (Cat#40041B, BioLegend, San Diego, CA 92121) was added to the medium.

Techniques: Co-Culture Assay, Derivative Assay, Cell Culture, Staining, Two Tailed Test