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Image Search Results
Journal: Cell reports
Article Title: Stromal-initiated changes in the bone promote metastatic niche development
doi: 10.1016/j.celrep.2015.12.016
Figure Lengend Snippet: A. Primary osteoblasts were obtained from the femurs of 6-week-old FASST mice. To activate the p27Kip1 transgene and induce senescence, FASST osteoblasts were treated with 10μM tamoxifen (4-OHT) for 3 days. SA-β-Gal staining was carried out on 4-OHT or vehicle (veh) treated osteoblasts on day 3. 4-OHT treatment resulted in a significant increase in enlarged, flattened cells that stained positive for SA-β-Gal (blue) compared to vehicle treated cells (lower left panel), scale bar is 200 μm (*p<0.05, two-tailed, Student t test. one of three representative experiments is shown). The induction of the senescence associated secretory phenotype (SASP) factor IL-6 was also quantitated by reverse transcriptase PCR (qRT-PCR) (lower right panel). Senescent osteoblasts expressed significantly more IL-6 mRNA compared to vehicle treated cells (SEM, *p<0.05, two-tailed, Student t test; one of three representative experiment is shown).
Article Snippet: For IL-6 neutralization in this assay, senescent or non-senescent osteoblasts were co-cultured with BMMs and 5pg/μl of
Techniques: Staining, Two Tailed Test, Quantitative RT-PCR
Journal: Cell reports
Article Title: Stromal-initiated changes in the bone promote metastatic niche development
doi: 10.1016/j.celrep.2015.12.016
Figure Lengend Snippet: A. Quantification of osteoclasts following co-culture with non-senescent (NS) or senescent (SEN) osteoblasts in the presence of a control (IgG) or IL-6 neutralizing antibody (α-IL-6). Bone marrow derived macrophages were cultured with non-senescent or senescent osteoblasts for 4 days in the presence of 5 pg α-IL-6. The antibody was replaced every other day. TRAP staining was carried out after 4 days co-culture and osteoclast numbers were quantitated and are displayed as TRAP-positive osteoclasts per field of view (TRAP+OC/FOV) (SEM, *p<0.05, two-tailed, Student t test; one of two representative experiment is shown).
Article Snippet: For IL-6 neutralization in this assay, senescent or non-senescent osteoblasts were co-cultured with BMMs and 5pg/μl of
Techniques: Co-Culture Assay, Derivative Assay, Cell Culture, Staining, Two Tailed Test